TB-500 Australia: Research Guide, Purity & COA
Published 9 June 2026
Compiled by the APL Research TeamSourced directly from peer-reviewed pharmacological literature and clinical guidelines.
Key Takeaways
- Expert Insight: TB-500 is a synthetic fragment of thymosin beta-4 (Tβ4) studied for its regulation of the actin cytoskeleton in cell-migration and wound-repair research.
- Quality Assurance: Every batch ships with a Certificate of Analysis (HPLC + MS); select batches receive independent third-party purity verification.
- Research Use Only: TB-500 is supplied for in-vitro laboratory research — not for human or animal consumption.
What Is TB-500?
TB-500 is a synthetic peptide corresponding to the active region (amino acids 17–23) of thymosin beta-4 (Tβ4), a 43-amino-acid protein naturally present in nearly all human and animal cells. Tβ4 was first isolated from the thymus gland in 1981 and is one of the most abundant intracellular peptides in mammalian tissue, with especially high concentrations in platelets, wound fluid, and developing tissue. The synthetic fragment retains the actin-binding and cell-signalling properties of the full-length protein while offering improved stability and ease of use in research settings.
Australian Peptide Labs supplies TB-500 in Australia as a research chemical for in-vitro laboratory work. This page is an overview and sourcing guide; for the underlying cell biology, see the deep-dive article linked below.
Molecular Profile
| Property | Value |
|---|---|
| Also Known As | Thymosin beta-4 fragment, Tβ4 |
| Type | Synthetic peptide fragment |
| Sequence (active region) | LKKTETQ |
| Full Tβ4 Molecular Formula | C₂₁₂H₃₅₀N₅₆O₇₈S |
| Full Tβ4 Molecular Weight | 4921.5 Da |
| Primary Intracellular Target | G-actin (monomeric actin) |
| Appearance | Lyophilised powder |
Research Applications
TB-500's research profile follows from its central biochemical role — sequestering G-actin (monomeric actin) and thereby regulating the actin cytoskeleton:
- Cell migration — by controlling actin-polymerisation dynamics, Tβ4 promotes the cell motility required for cells at a wound margin to migrate into the wound bed.
- Wound-repair models — studied for effects on granulation, re-epithelialisation, and tissue regeneration.
- Anti-inflammatory pathways — downregulation of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and suppression of NF-κB signalling in culture models.
These are preclinical and in-vitro findings only and do not establish any effect in humans. For the full mechanistic detail — including actin regulation and angiogenic pathways — see our deep-dive article: TB-500 (Thymosin Beta-4): Structure, Function, and Research Applications. Researchers studying combined tissue-repair models may also be interested in BPC-157 and TB-500: Complementary Mechanisms in Tissue Repair Research.
Quality & Certificate of Analysis
Every batch of TB-500 is analysed in-house by high-performance liquid chromatography (HPLC) and mass spectrometry (MS), and ships with a batch-specific Certificate of Analysis (COA). HPLC quantifies purity (we specify ≥98%, the High-Tier Research Grade standard), while MS confirms the molecular identity of the peptide. Select batches also undergo independent, third-party purity verification.
Learn more: Every TB-500 batch certificate · How purity is verified · How to read a COA.
Handling, Reconstitution & Storage
TB-500 is supplied lyophilised. Store the lyophilised powder at -20°C, reconstitute with bacteriostatic water following standard protocols, and keep the reconstituted solution refrigerated (2-8°C), protected from light, with freeze-thaw cycles minimised.
Sourcing TB-500 for Research in Australia
Australian Peptide Labs supplies TB-500 as a research chemical for in-vitro laboratory use only. Orders are dispatched same day from within Australia with a Certificate of Analysis included, with cold-chain handling where required. TB-500 is not approved for human therapeutic use and is not for human or animal consumption; researchers are responsible for compliance with TGA regulations and institutional protocols.
View TB-500 product details & current purity →
Frequently Asked Questions
Is TB-500 legal to buy in Australia? TB-500 is supplied strictly as a research chemical for in-vitro laboratory use. It is not approved for human therapeutic use in Australia and is not for human or animal consumption. Researchers are responsible for compliance with Therapeutic Goods Administration (TGA) regulations and their institution's protocols.
How is the purity of TB-500 verified? Every batch is analysed in-house by high-performance liquid chromatography (HPLC) and mass spectrometry (MS) and ships with a Certificate of Analysis. Select batches also undergo independent, third-party purity verification.
What is TB-500 studied for in research? TB-500 is a synthetic fragment of thymosin beta-4 studied in cell-migration, wound-repair, and anti-inflammatory research models, largely through its regulation of the actin cytoskeleton. These are laboratory research findings only and do not establish any effect in humans.
Does Australian Peptide Labs provide dosing guidance? No. As these compounds are supplied for laboratory research only, we do not provide dosing or administration protocols. Our research library covers reconstitution and concentration calculations for in-vitro work.
How should TB-500 be stored and reconstituted? TB-500 ships as a lyophilised powder and is stable at -20°C before reconstitution. Reconstitute with bacteriostatic water following standard protocols, then store the solution at 2-8°C, protected from light, and minimise freeze-thaw cycles. See our reconstitution and storage guides for full protocols.
References
- Goldstein, A.L., Hannappel, E., Kleinman, H.K "Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues." Trends in Molecular Medicine, 2005. — PubMed: 16099219
- Malinda, K.M. et al. "Thymosin beta4 accelerates wound healing." The Journal of Investigative Dermatology, 1999. — PubMed: 10469335
- Bock-Marquette, I. et al. "Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair." Nature, 2004. — PubMed: 15565145
- Philp, D., Kleinman, H.K "Animal studies with thymosin beta, a multifunctional tissue repair and regeneration peptide." Annals of the New York Academy of Sciences, 2010. — PubMed: 20536453
⚠️ Medical & Regulatory Disclaimer:The information provided in this academic article is intended exclusively for educational and laboratory research purposes. It does NOT constitute medical advice. Compounds discussed are strictly for in-vitro research and development only, and are not intended for human consumption, veterinary use, or clinical treatment. Always adhere to Australian Therapeutic Goods Administration (TGA) regulations and your institution's ethical guidelines when handling research chemicals.